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    BIOSENSORS

    GENERAL PRINCIPLE

    This technology consists in a set of more than 25 BioSensor assays, each designed to follow one specific signaling pathway. This BRET-based BioSensor platform was implemented to interrogate the signaling complexity associated with GPCR activation and is the tool of choice for the identification of biased ligands.

    BioSensor

    BioSensors CAN BE DIVIDED IN TWO FAMILIES

    Unimolecular BRET-based BioSensors that are based on the detection of intramolecular rearrangements of a sensor attached to both energy donor and acceptor that is promoted either by the binding of a second messenger or partner protein, a translocation event from the cytoplasm to the plasma membrane or a phosphorylation.
    The example presents a BioSensor that detects the production of cAMP. Binding of the receptor to the BioSensor leads to a conformational rearrangement that changes the distance between the energy donor luciferase (Luc) and acceptor GFP thus yielding a change in BRET.

    Bimolecular BRET-based BioSensors that are based on protein–protein interactions that are modified by a signaling event.
    The example presents a BioSensor monitoring the activation of a G-protein by assessing the separation between the Gα and Gγ subunits that follows G-protein activation and results in a BRET change.

    BioSensor